The structure below shows pUC18 which is similar to pBR322 in its function. However, they differ in some of their restriction sites and number of ori. The ori number for pBR322 is approximately 20.



a) How are puc18 and pBR322 used in biotechnological studies?

OR

What will be the impact if oriin the above structure gets damaged?
b) The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions

5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’

i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason.
c) Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.

Solution