(i) Name the source of thermostable DNA polymerase
(ii) Mention the scientific name of protozoan parasite that causes Amoebiasis
(iii) What is cryopreservation
(iv) The maternal grandfather of a boy is colourblind, but his maternal grandmother is normal. The father of the boy is also normal. What is the probability of this boy being colourblind?
(v) Name the type of antibody that can be transferred through the placenta.
(vi) During which phase of the cell cycle, does DNA replication take place?
(vii) How many sets of primers are required in each cycle of PCR?
(viii) Define perisperm.

Solution

(i) Thermus aquaticus
(ii) Ascaris lumbricoides
(iii) Cryopreservation is the method of storage of materials at ultra-low temperature either by very rapid cooling or by gradual cooling and simultaneous dehydration at low temperature.
(iv) According to the given information: Maternal grandfather of a boy is colour blind. Therefore, his daughter(Mother of the boy) would be carrier for colour blindness. Genotype would be X°X. On the other hand, father of a boy is normal with genotype (XY). So, the boy has 50% probability of being colour blind.
(v) IgG is the antibody can be transferred through the placenta.
(vi) S- Phase of the interphase period.
(vii) Two sets of primers are required in each cycle of PCR (Polymerase Chain Reaction) as primers are necessary to start functioning of DNA polymerase.
(viii) The perisperm is the nucellus that remains after fertilisation. They are the nutritive tissues deposited external to the embryo.